The solution that we use are described in the original microisland paper Segal and Furshpan (1990) in J. Neurophysiology or in a later paper Segal (1994) in J. Neurophysiology. The solutions are described in more detail in links from this page. Many of the solutions are based on recipes from the Furshpan and Potter labs; the recipes given here are the versions we use now. The recipes are given using a huge amount of detail so they can be used by a person with little experience in culturing.
If any of these recipes are unclear or if you need further recipes please let me know.
Copyright © 2002 Dr. Michael M. Segal, Department of Neurosurgery, Brigham & Women's Hospital, Boston MA 02115, USA. Individual copies may be made of this page for use in educational institutions.