STERILE (no filtering needed since all ingredients are sterile)
For 250 ml:
INGREDIENT | ml per 250 ml | Final solution has mM: |
Na2SO4 1 M | 22.50 | 90.00 |
K2SO4 0.25 M | 30.00 | 30.00 |
MgCl2 1 M | 1.45 | 5.80 |
CaCl2 0.1 M | 6.20 | 0.25 |
Sodium HEPES 500 mM | 0.50 | 1.00 (has 0.4 Na+) |
Sodium phenol red | 0.20 | N/A |
Autoclaved water | Fill to 250 ml |
Do not filter since all the ingredients are sterile.
Copyright © 2002 Dr. Michael M. Segal, Department of Neurosurgery, Brigham & Women's Hospital, Boston MA 02115, USA. Many of the solutions are based on recipes from the Furshpan and Potter labs; the recipes given here are the versions we use now in the Segal lab. Individual copies may be made of this page for use in educational institutions.