STERILE (no filtering needed since all ingredients are sterile)
For 250 ml:
|INGREDIENT||ml per 250 ml||Final solution has mM:|
|Na2SO4 1 M||22.50||90.00|
|K2SO4 0.25 M||30.00||30.00|
|MgCl2 1 M||1.45||5.80|
|CaCl2 0.1 M||6.20||0.25|
|Sodium HEPES 500 mM||0.50||1.00
(has 0.4 Na+)
|Sodium phenol red||0.20||N/A|
|Autoclaved water||Fill to 250 ml|
Do not filter since all the ingredients are sterile.
Copyright © 2002 Dr. Michael M. Segal, Department of Neurosurgery, Brigham & Women's Hospital, Boston MA 02115, USA. Many of the solutions are based on recipes from the Furshpan and Potter labs; the recipes given here are the versions we use now in the Segal lab. Individual copies may be made of this page for use in educational institutions.