Stable vitamins and other nutrients, modified to exclude glutamate, aspartate and
Identical to the "Stable Vitamin Mix" recipe from the Furshpan lab.
WARNING: Some of the ingredients in this mixture do not dissolve. This problem particularly applies to the biotin-thiotic acid mixture - when adding this mixture, it is important to have just shaken that suspension before pipetting several milliliters out from the middle of the tube. Also, when preparing 10 ml aliquots from the beaker to put into 13 ml test tubes, it is important to keep the mixture stirring with an electric stirrer so as to keep the solid material in suspension.
WARNING: Choline chloride can absorb water if stored improperly. If it is wet or very lumpy, it should not be used.
Equipment and supplies:
Storage containers: 13 ml plastic tubes, labeled "mod SVM".
Vitamin additive mix :Add the following to the glass beaker:
|INGREDIENT||SOURCE||For 200 ml||For 400 ml|
|Distilled water||198 ml||396 ml|
|L-proline||Sigma P-0380||600 mg||1200 mg|
|L-cystine||Sigma C-8755||600 mg||1200 mg|
(GABA free acid, crystalline)
|Sigma A-9878||200 mg||400 mg|
|vitamin B-12||Sigma V-2876||80 mg||160 mg|
|i-Inositol (meso)||Sigma I-5125||400 mg||800 mg|
|choline chloride, crystalline||Sigma C-1879||400 mg||800 mg|
|fumaric acid||Sigma F-2752||1000 mg||2000 mg|
|coenzyme A||P-L Biochemicals # 8300A||16 mg||32 mg|
|Solution from the biotin - thiotic acid suspension in the 13 ml tube just shaken to suspend the ingredients||2 ml||4 ml|
|Leave this stirring automatically about 2 hours before putting 10 ml into each 13 ml tube.
Biotin - thiotic acid suspension: Add the following to the 13 ml tube:
|Distilled water||10 ml|
|D-biotin||Sigma B-4501||0.4 mg|
|DL-6,8-thiotic acid, lyophilized||Sigma T-5625||100 mg|
P-L Biochemicals is 1037 West McKinlez, Milwaukee WI 53205, 800 558-7110.
D-biotin is sometimes called vitamin H.
Thiotic acid is sometimes called lipoic acid.
Copyright © 2002 Dr. Michael M. Segal, Department of Neurosurgery, Brigham & Women's Hospital, Boston MA 02115, USA. Many of the solutions are based on recipes from the Furshpan and Potter labs; the recipes given here are the versions we use now in the Segal lab. Individual copies may be made of this page for use in educational institutions.